Diversity and evolution of chitin synthases in oomycetes (Straminipila: Oomycota).
Identifieur interne : 000535 ( Main/Exploration ); précédent : 000534; suivant : 000536Diversity and evolution of chitin synthases in oomycetes (Straminipila: Oomycota).
Auteurs : Stefan Klinter [Suède] ; Vincent Bulone [Australie] ; Lars Arvestad [Suède]Source :
- Molecular phylogenetics and evolution [ 1095-9513 ] ; 2019.
Descripteurs français
- KwdFr :
- Chitine synthase (composition chimique), Chitine synthase (génétique), Domaines protéiques (MeSH), Fonctions de vraisemblance (MeSH), Oomycetes (enzymologie), Oomycetes (génétique), Phylogenèse (MeSH), Séquence conservée (génétique), Séquence d'acides aminés (MeSH), Variation génétique (MeSH), Évolution moléculaire (MeSH).
- MESH :
- composition chimique : Chitine synthase.
- enzymologie : Oomycetes.
- génétique : Chitine synthase, Oomycetes, Séquence conservée.
- Domaines protéiques, Fonctions de vraisemblance, Phylogenèse, Séquence d'acides aminés, Variation génétique, Évolution moléculaire.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Chitin Synthase.
- chemical , genetics : Chitin Synthase.
- enzymology : Oomycetes.
- genetics : Conserved Sequence, Oomycetes.
- Amino Acid Sequence, Evolution, Molecular, Genetic Variation, Likelihood Functions, Phylogeny, Protein Domains.
Abstract
The oomycetes are filamentous eukaryotic microorganisms, distinct from true fungi, many of which act as crop or fish pathogens that cause devastating losses in agriculture and aquaculture. Chitin is present in all true fungi, but it occurs in only small amounts in some Saprolegniomycetes and it is absent in Peronosporomycetes. However, the growth of several oomycetes is severely impacted by competitive chitin synthase (CHS) inhibitors. Here, we shed light on the diversity, evolution and function of oomycete CHS proteins. We show by phylogenetic analysis of 93 putative CHSs from 48 highly diverse oomycetes, including the early diverging Eurychasma dicksonii, that all available oomycete genomes contain at least one putative CHS gene. All gene products contain conserved CHS motifs essential for enzymatic activity and form two Peronosporomycete-specific and six Saprolegniale-specific clades. Proteins of all clades, except one, contain an N-terminal microtubule interacting and trafficking (MIT) domain as predicted by protein domain databases or manual analysis, which is supported by homology modelling and comparison of conserved structural features from sequence logos. We identified at least three groups of CHSs conserved among all oomycete lineages and used phylogenetic reconciliation analysis to infer the dynamic evolution of CHSs in oomycetes. The evolutionary aspects of CHS diversity in modern-day oomycetes are discussed. In addition, we observed hyphal tip rupture in Phytophthora infestans upon treatment with the CHS inhibitor nikkomycin Z. Combining data on phylogeny, gene expression, and response to CHS inhibitors, we propose the association of different CHS clades with certain developmental stages.
DOI: 10.1016/j.ympev.2019.106558
PubMed: 31288106
Affiliations:
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Chitin is present in all true fungi, but it occurs in only small amounts in some Saprolegniomycetes and it is absent in Peronosporomycetes. However, the growth of several oomycetes is severely impacted by competitive chitin synthase (CHS) inhibitors. Here, we shed light on the diversity, evolution and function of oomycete CHS proteins. We show by phylogenetic analysis of 93 putative CHSs from 48 highly diverse oomycetes, including the early diverging Eurychasma dicksonii, that all available oomycete genomes contain at least one putative CHS gene. All gene products contain conserved CHS motifs essential for enzymatic activity and form two Peronosporomycete-specific and six Saprolegniale-specific clades. Proteins of all clades, except one, contain an N-terminal microtubule interacting and trafficking (MIT) domain as predicted by protein domain databases or manual analysis, which is supported by homology modelling and comparison of conserved structural features from sequence logos. We identified at least three groups of CHSs conserved among all oomycete lineages and used phylogenetic reconciliation analysis to infer the dynamic evolution of CHSs in oomycetes. The evolutionary aspects of CHS diversity in modern-day oomycetes are discussed. In addition, we observed hyphal tip rupture in Phytophthora infestans upon treatment with the CHS inhibitor nikkomycin Z. Combining data on phylogeny, gene expression, and response to CHS inhibitors, we propose the association of different CHS clades with certain developmental stages.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">31288106</PMID><DateCompleted><Year>2020</Year><Month>03</Month><Day>06</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>06</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1095-9513</ISSN><JournalIssue CitedMedium="Internet"><Volume>139</Volume><PubDate><Year>2019</Year><Month>10</Month></PubDate></JournalIssue><Title>Molecular phylogenetics and evolution</Title><ISOAbbreviation>Mol Phylogenet Evol</ISOAbbreviation></Journal><ArticleTitle>Diversity and evolution of chitin synthases in oomycetes (Straminipila: Oomycota).</ArticleTitle><Pagination><MedlinePgn>106558</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S1055-7903(18)30183-0</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ympev.2019.106558</ELocationID><Abstract><AbstractText>The oomycetes are filamentous eukaryotic microorganisms, distinct from true fungi, many of which act as crop or fish pathogens that cause devastating losses in agriculture and aquaculture. Chitin is present in all true fungi, but it occurs in only small amounts in some Saprolegniomycetes and it is absent in Peronosporomycetes. However, the growth of several oomycetes is severely impacted by competitive chitin synthase (CHS) inhibitors. Here, we shed light on the diversity, evolution and function of oomycete CHS proteins. We show by phylogenetic analysis of 93 putative CHSs from 48 highly diverse oomycetes, including the early diverging Eurychasma dicksonii, that all available oomycete genomes contain at least one putative CHS gene. All gene products contain conserved CHS motifs essential for enzymatic activity and form two Peronosporomycete-specific and six Saprolegniale-specific clades. Proteins of all clades, except one, contain an N-terminal microtubule interacting and trafficking (MIT) domain as predicted by protein domain databases or manual analysis, which is supported by homology modelling and comparison of conserved structural features from sequence logos. We identified at least three groups of CHSs conserved among all oomycete lineages and used phylogenetic reconciliation analysis to infer the dynamic evolution of CHSs in oomycetes. The evolutionary aspects of CHS diversity in modern-day oomycetes are discussed. In addition, we observed hyphal tip rupture in Phytophthora infestans upon treatment with the CHS inhibitor nikkomycin Z. Combining data on phylogeny, gene expression, and response to CHS inhibitors, we propose the association of different CHS clades with certain developmental stages.</AbstractText><CopyrightInformation>Copyright © 2019 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Klinter</LastName><ForeName>Stefan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>KTH Royal Institute of Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health, Department of Chemistry, Division of Glycoscience, AlbaNova University Centre, 100 44 Stockholm, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bulone</LastName><ForeName>Vincent</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>KTH Royal Institute of Technology, School of Engineering Sciences in Chemistry, Biotechnology and Health, Department of Chemistry, Division of Glycoscience, AlbaNova University Centre, 100 44 Stockholm, Sweden; ARC Centre of Excellence in Plant Cell Walls and School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, Urrbrae, SA 5064, Australia. Electronic address: bulone@kth.se.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Arvestad</LastName><ForeName>Lars</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Mathematics, Stockholm University, Swedish e-Science Research Centre, Science for Life Laboratory, 106 91 Stockholm, Sweden. Electronic address: arve@math.su.se.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>07</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Phylogenet Evol</MedlineTA><NlmUniqueID>9304400</NlmUniqueID><ISSNLinking>1055-7903</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>EC 2.4.1.16</RegistryNumber><NameOfSubstance UI="D002687">Chitin Synthase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002687" MajorTopicYN="N">Chitin Synthase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017124" MajorTopicYN="N">Conserved Sequence</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019143" MajorTopicYN="Y">Evolution, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="Y">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016013" MajorTopicYN="N">Likelihood Functions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009868" MajorTopicYN="N">Oomycetes</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000072417" MajorTopicYN="N">Protein Domains</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Chitin synthase</Keyword><Keyword MajorTopicYN="Y">Evolution</Keyword><Keyword MajorTopicYN="Y">Growth inhibition</Keyword><Keyword MajorTopicYN="Y">Microtubule interacting and trafficking (MIT) domain</Keyword><Keyword MajorTopicYN="Y">Oomycete</Keyword><Keyword MajorTopicYN="Y">Phylogeny</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>04</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>07</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>07</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>7</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>3</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>7</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31288106</ArticleId><ArticleId IdType="pii">S1055-7903(18)30183-0</ArticleId><ArticleId IdType="doi">10.1016/j.ympev.2019.106558</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Australie</li><li>Suède</li></country><region><li>Comté de Stockholm</li><li>Svealand</li></region><settlement><li>Stockholm</li></settlement><orgName><li>Université de Stockholm</li></orgName></list><tree><country name="Suède"><noRegion><name sortKey="Klinter, Stefan" sort="Klinter, Stefan" uniqKey="Klinter S" first="Stefan" last="Klinter">Stefan Klinter</name></noRegion><name sortKey="Arvestad, Lars" sort="Arvestad, Lars" uniqKey="Arvestad L" first="Lars" last="Arvestad">Lars Arvestad</name></country><country name="Australie"><noRegion><name sortKey="Bulone, Vincent" sort="Bulone, Vincent" uniqKey="Bulone V" first="Vincent" last="Bulone">Vincent Bulone</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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